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Allergol Int. 2010 Jun;59(2):223-8. doi: 10.2332/allergolint.09-OA-0161. Epub 2010 Apr 24.

Detection of indoor and outdoor avian antigen in management of bird-related hypersensitivity pneumonitis.

Author information

1
Department of Integrated Pulmonology, Tokyo Medical and Dental University, Tokyo, Japan.

Abstract

BACKGROUND:

In the management of hypersensitivity pneumonitis (HP), antigen avoidance is crucial to prevent the progression of disease. Indirect and unrecognized exposure to the antigen may continue for a long time if persistence of the causative antigen is not recognized. To make a correct assessment of the patients' environment, we tried to establish the methods to detect indoor and outdoor avian antigens.

METHODS:

Sixteen patients with bird-related HP, 4 asymptomatic breeders, and 6 healthy controls were examined. We prepared anti-pigeon dropping extracts (PDE) polyclonal antibody from rabbits. Air samples and house dust samples were analyzed by an antigen-capture ELISA with signal amplification using catalyzed reporter deposition.

RESULTS:

In air samples, avian antigen could be detected in patients with HP (0.73 +/- 0.53 ng/m3) and asymptomatic breeders (0.63 +/- 0.23 ng/m3). In house dust samples, the amount of avian antigen was higher in patients with HP (2.4 +/- 1.8 microg/g) and asymptomatic breeders (4.1 +/- 2.3 microg/g) than in the controls (0.1 +/- 0.2 microg/g).

CONCLUSIONS:

Detection of indoor and outdoor avian antigen might contribute to the correct diagnosis and appropriate managements of bird-related HP.

PMID:
20414051
DOI:
10.2332/allergolint.09-OA-0161
[Indexed for MEDLINE]
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