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Biophys J. 2010 Apr 21;98(8):L29-31. doi: 10.1016/j.bpj.2010.02.028.

Myosin IIA dependent retrograde flow drives 3D cell migration.

Abstract

Epithelial cell migration is an essential part of embryogenesis and tissue regeneration, yet their migration is least understood. Using our three-dimensional (3D) motility analysis, migrating epithelial cells formed an atypical polarized cell shape with the nucleus leading the cell front and a contractile cell rear. Migrating epithelial cells exerted traction forces to deform both the anterior and posterior extracellular matrix toward the cell body. The cell leading edge exhibited a myosin II-dependent retrograde flow with the magnitude and direction consistent with surrounding network deformation. Interestingly, on a two-dimensional substrate, myosin IIA-deficient cells migrated faster than wild-type cells, but in a 3D gel, these myosin IIA-deficient cells were unpolarized and immobile. In contrast, the migration rates of myosin IIB-deficient cells were similar to wild-type cells. Therefore, myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.

PMID:
20409454
PMCID:
PMC2856159
DOI:
10.1016/j.bpj.2010.02.028
[Indexed for MEDLINE]
Free PMC Article

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