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Cell Cycle. 2010 May;9(9):1802-8. Epub 2010 May 15.

High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.

Author information

1
Max F. Perutz Laboratories, Department of Chromosome Biology, University of Vienna, Vienna, Austria.

Abstract

Meiosis is the process which produces haploid gametes from diploid precursor cells. This reduction of chromosome number is achieved by two successive divisions. Whereas homologs segregate during meiosis I, sister chromatids segregate during meiosis II. To identify novel proteins required for proper segregation of chromosomes during meiosis, we applied a high-throughput knockout technique to delete 87 S. pombe genes whose expression is upregulated during meiosis and analyzed the mutant phenotypes. Using this approach, we identified a new protein, Dil1, which is required to prevent meiosis I homolog non-disjunction. We show that Dil1 acts in the dynein pathway to promote oscillatory nuclear movement during meiosis.

PMID:
20404563
PMCID:
PMC2883734
DOI:
10.4161/cc.9.9.11526
[Indexed for MEDLINE]
Free PMC Article

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