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J Pediatr Gastroenterol Nutr. 2010 Jun;50(6):691-3. doi: 10.1097/MPG.0b013e3181cd26e5.

Bilirubin, a physiological antioxidant, can improve cryopreservation of human hepatocytes.

Author information

1
Laboratory of Medicine, Ospedale Bambino Gesù, Rome, Italy.

Abstract

The availability of cryopreserved hepatocytes is required for a more widespread use of hepatocyte transplantation, but human hepatocytes are easily damaged during freezing-thawing. Here, preincubation with unconjugated bilirubin, a physiological antioxidant, resulted in increased viability and function of hepatocytes (as determined by trypan blue exclusion, mitochondrial succinate dehydrogenases activity, urea synthesis, and cytochrome P450 1A/2) compared with cells incubated without the pigment. These findings suggest that unconjugated bilirubin may be used as cryoprotectant in clinical hepatocyte transplantation.

PMID:
20400908
DOI:
10.1097/MPG.0b013e3181cd26e5
[Indexed for MEDLINE]

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