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Clin Biochem. 2010 Jul;43(10-11):822-6. doi: 10.1016/j.clinbiochem.2010.04.005. Epub 2010 Apr 14.

Hyaluronic acid signals for repair in ethanol-induced apoptosis in skin cells in vitro.

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Department of Clinical Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada M56 1L7.


Ethanol is commonly used in cosmetic and pharmaceutical preparations.


The present study aimed to assess ethanol-induced apoptosis and the possible repair by hyaluronic acid (HA) in vitro. In addition we aimed to determine the modulation of tumor necrosis factor (TNF-alpha) and interferon (IFN-alpha).


We treated human A431 epidermoid skin cells and mouse fibroblasts with two concentrations of ethanol for 24h. HA obtained from umbilical cord excision was used at three concentration levels (2%, 4% and 8%) to determine its efficacy in the treatment. We measured cytotoxicity, TNF-alpha and IFN-alpha and visualized the cultures by electron microscopy.


Treatment of cells with ethanol at 50mM and 100mM increased both the percentage of cells undergoing apoptosis, as well as the release of TNF-alpha into the culture medium.


Ethanol may induce apoptosis in skin cells by enhancing the effects of TNF-alpha. HA in the 2% and 4% concentrations reduced TNF-alpha and morphological inflammation both in human A431 epidermoid skin cells and in mouse fibroblasts.

[Indexed for MEDLINE]

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