Format

Send to

Choose Destination
Curr Protoc Protein Sci. 2010 Apr;Chapter 7:Unit 7.13. doi: 10.1002/0471140864.ps0713s60.

Methods for the design and analysis of sedimentation velocity and sedimentation equilibrium experiments with proteins.

Author information

1
Department of Biochemistry, The University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA.

Abstract

Analytical ultracentrifugation experiments play an integral role in the solution phase characterization of recombinant proteins and other biological macromolecules. This unit discusses the design of sedimentation velocity and sedimentation equilibrium experiments performed with a Beckman Optima XL-A or XL-I analytical ultracentrifuge. Optimal instrument settings and experimental design considerations are explained, and strategies for the analysis of experimental data with the UltraScan data analysis software package are presented. Special attention is paid to the strengths and weaknesses of the available detectors, and guidance is provided on how to extract maximum information from analytical ultracentrifugation experiments.

PMID:
20393977
PMCID:
PMC4547541
DOI:
10.1002/0471140864.ps0713s60
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center