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J Clin Microbiol. 2010 Jun;48(6):2240-2. doi: 10.1128/JCM.00553-10. Epub 2010 Apr 14.

Rapid diagnosis of Old World Leishmaniasis by high-resolution melting analysis of the 7SL RNA gene.

Author information

1
Department of Microbiology and Molecular Genetics, IMIRC, the Kuvin Center for the Study of Infectious and Tropical Diseases, the Hebrew University-Hadassah Medical School, P.O. Box 12272, Jerusalem 91120, Israel.

Abstract

High-resolution melt analysis PCR (HRM PCR) for diagnosis of Old World Leishmania was developed using the 7SL RNA gene. Cutaneous leishmaniasis samples were analyzed. Sensitivity and specificity of HRM PCR were significantly better (P < 0.001) than those of internal transcribed spacer 1 PCR and similar to those of kinetoplast DNA PCR.

PMID:
20392923
PMCID:
PMC2884505
DOI:
10.1128/JCM.00553-10
[Indexed for MEDLINE]
Free PMC Article

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