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Lab Chip. 2010 May 7;10(9):1113-9. doi: 10.1039/b922884e. Epub 2010 Mar 16.

Microfluidic image cytometry for quantitative single-cell profiling of human pluripotent stem cells in chemically defined conditions.

Author information

1
Department of Molecular & Medical Pharmacology, University of California, Los Angeles, CA 90095, USA. kkamei@mednet.ucla.edu

Abstract

Microfluidic image cytometry (MIC) has been developed to study phenotypes of various hPSC lines by screening several chemically defined serum/feeder-free conditions. A chemically defined hPSC culture was established using 20 ng mL(-1) of bFGF on 20 microg mL(-1) of Matrigel to grow hPSCs over a week in an undifferentiated state. Following hPSC culture, we conducted quantitative MIC to perform a single cell profiling of simultaneously detected protein expression (OCT4 and SSEA1). Using clustering analysis, we were able to systematically compare the characteristics of various hPSC lines in different conditions.

PMID:
20390128
PMCID:
PMC2970622
DOI:
10.1039/b922884e
[Indexed for MEDLINE]
Free PMC Article

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