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Nano Lett. 2010 May 12;10(5):1823-30. doi: 10.1021/nl100533c.

Probing cellular traction forces by micropillar arrays: contribution of substrate warping to pillar deflection.

Author information

1
Laboratory for Biologically Oriented Materials, Department of Materials, ETH Zurich, Zurich, Switzerland.

Abstract

Quantifying cellular forces relies on accurate calibrations of the sensor stiffness. Neglecting deformations of elastic substrates to which elastic pillars are anchored systematically overestimates the applied forces (up to 40%). A correction factor considering substrate warping is derived analytically and verified experimentally. The factor scales with the dimensionless pillar aspect ratio. This has significant implications when designing pillar arrays or comparing absolute forces measured on different pillar geometries during cell spreading, motility, or rigidity sensing.

PMID:
20387859
PMCID:
PMC2881340
DOI:
10.1021/nl100533c
[Indexed for MEDLINE]
Free PMC Article

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