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PLoS One. 2010 Apr 8;5(4):e10079. doi: 10.1371/journal.pone.0010079.

A proteomic approach for the diagnosis of bacterial meningitis.

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1
Department of Neurology, University of Ulm, Ulm, Germany.

Abstract

BACKGROUND:

The discrimination of bacterial meningitis (BM) versus viral meningitis (VM) shapes up as a problem, when laboratory data are not equivocal, in particular, when Gram stain is negative.

METHODOLOGY/PRINCIPAL FINDINGS:

With the aim to determine reliable marker for bacterial or viral meningitis, we subjected cerebrospinal fluid (CSF) to a quantitative proteomic screening. By using a recently established 2D-DIGE protocol which was adapted to the individual CSF flow, we compared a small set of patients with proven BM and VM. Thereby, we identified six potential biomarkers out of which Prostaglandin-H2 D-isomerase was already described in BM, showing proof of concept. In the subsequent validation phase on a more comprehensive collective of 80 patients, we could validate that in BM high levels of glial fibrillary acidic protein (GFAP) and low levels of soluble amyloid precursor protein alpha/beta (sAPPalpha/beta) are present as possible binding partner of Fibulin-1.

CONCLUSIONS/SIGNIFICANCE:

We conclude that our CSF flow-adapted 2D-DIGE protocol is valid especially in comparing samples with high differences in total protein and suppose that GFAP and sAPPalpha/beta have a high potential as additional diagnostic markers for differentiation of BM from VM. In the clinical setting, this might lead to an improved early diagnosis and to an individual therapy.

PMID:
20386697
PMCID:
PMC2851643
DOI:
10.1371/journal.pone.0010079
[Indexed for MEDLINE]
Free PMC Article
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