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Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Feb;30(2):372-5.

[SERS spectra of Xanthomonas oryzae pv. Oryzae (Xoo) on nano silver film prepared by electrolysis method].

[Article in Chinese]

Author information

1
Department of Physics and Electron Science, Chuxiong Normal University, Chuxiong 675000, China. kangyipu@hotmail.com

Abstract

The nano silver film was prepared by electrolysis method using silver nitrate and polyvinyl alcohol (PVA) in deionized water as the electrolyte, with four glass slides put in the electrolyte and two silver rods dipped into the electrolyte as the anode and cathode. A direct current was applied to the rods, then the four glass slides stayed in the silver colloids. Thus the authors got the nano silver film. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to detect the silver particles in the silver colloids and on the nano silver film. From the SEM we can see that the silver particles on the film formed different layers. In one layer, the distance between two particles was about 100 nm. The samples of Xanthomonas oryzae pv. Oryzae (Xoo) were 7 different kinds of bacterial blight, namely 1-YN1, 2-YN7, 3-YN11, 4-GD414, 5-SCYC6, 6-HEN11 and 7-FWJ. Because the silver particles in the colloids were aggregated on the film, there was large electromagnetic potentiation. So the SERS spectra of Xoo were perfect. The authors used the area analytical method to distinguish the different kinds of Xoo. The silver film prepared by electrolysis was cheap and active, the preparation time of the samples was short, and any normal chemistry lab can make it, which can find excellent application to detecting the Xoo in agriculture. On the other hand, this film is active on biomolecules and bioorganism, which may be a new kind of SERS fundus to explain the creation of the SERS. Further study was under way.

PMID:
20384127
[Indexed for MEDLINE]

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