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Nat Methods. 2010 May;7(5):395-7. doi: 10.1038/nmeth.1448. Epub 2010 Apr 11.

In situ detection and genotyping of individual mRNA molecules.

Author information

1
Department of Genetics and Pathology, Uppsala University, Sweden.

Abstract

Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.

PMID:
20383134
DOI:
10.1038/nmeth.1448
[Indexed for MEDLINE]

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