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Mol Imaging Biol. 2011 Feb;13(1):3-9. doi: 10.1007/s11307-010-0322-0.

Labeling human mesenchymal stem cells with fluorescent contrast agents: the biological impact.

Author information

  • 1Department of Radiology, University of California, San Francisco, San Francisco, CA, USA. Sophie.Boddington@radiology.ucsf.edu

Abstract

PURPOSE:

This study aims to determine the effect of human mesenchymal stem cell (hMSC) labeling with the fluorescent dye DiD and the iron oxide nanoparticle ferucarbotran on chondrogenesis.

PROCEDURES:

hMSCs were labeled with DiD alone or with DiD and ferucarbotran (DiD/ferucarbotran). hMSCs underwent confocal microscopy, optical imaging (OI), and magnetic resonance (MR) imaging. Chondrogenesis was induced by transforming growth factor-b and confirmed by histopathology and glycosaminoglycan (GAG) production. Data of labeled and unlabeled hMSCs were compared with a t test.

RESULTS:

Cellular uptake of DiD and ferucarbotran was confirmed with confocal microscopy. DiD labeling caused a significant fluorescence on OI, and ferucarbotran labeling caused a significant T2* effect on MR images. Compared to nonlabeled controls, progenies of labeled MSCs exhibited similar chondrocyte morphology after chondrogenic differentiation, but the labeled cells demonstrated significantly reduced GAG production (p < 0.05).

CONCLUSION:

DiD and DiD/ferucarbotran labeling of hMSC does not interfere with cell viability or morphologic differentiation into chondrocytes, but labeled cells exhibit significantly less GAG production compared to unlabeled cells.

PMID:
20379785
PMCID:
PMC3023037
DOI:
10.1007/s11307-010-0322-0
[PubMed - indexed for MEDLINE]
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