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FEMS Microbiol Lett. 1991 Jan 15;61(2-3):199-203.

Development and evaluation of the polymerase chain reaction to detect Mycoplasma genitalium.

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Division of Sexually Transmitted Diseases, Clinical Research Centre, Harrow, Middlesex, U.K.


The polymerase chain reaction (PCR) was developed to detect Mycoplasma genitalium. Oligonucleotide primers were used to amplify a 374 bp region of the attachment protein of the mycoplasma. DNA from three strains of M. genitalium tested gave a characteristic PCR product which was not seen with DNA from any other source. As little as 10(-15) g of M. genitalium DNA could be detected and it was found in the vagina of progesterone-treated BALB/c mice inoculated with M. genitalium organisms later than they could be cultured from this site, but not in mice that never became colonised vaginally.

[Indexed for MEDLINE]

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