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PLoS One. 2010 Apr 2;5(4):e10011. doi: 10.1371/journal.pone.0010011.

Enhanced beetle luciferase for high-resolution bioluminescence imaging.

Author information

1
National Institute of Advanced Industrial Science and Technology, Ikeda, Osaka, Japan. y-nakajima@aist.go.jp

Abstract

We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed that ELuc produces a strong light signal in primary cells and tissues and that it enables the visualization of gene expression with high temporal resolution at the single-cell level. Moreover, we successfully imaged the nucleocytoplasmic shuttling of importin alpha by fusing ELuc at the intracellular level. These results demonstrate that the use of ELuc allows a BLI spatiotemporal resolution far greater than that provided by FLuc.

PMID:
20368807
PMCID:
PMC2848861
DOI:
10.1371/journal.pone.0010011
[Indexed for MEDLINE]
Free PMC Article

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