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Nat Chem Biol. 2010 May;6(5):324-6. doi: 10.1038/nchembio.348. Epub 2010 Apr 4.

Activation of membrane-permeant caged PtdIns(3)P induces endosomal fusion in cells.

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1
European Molecular Biology Institute, Cell Biology and Biophysics Unit, Heidelberg, Germany.

Abstract

Phosphatidylinositol 3-phosphate (PtdIns(3)P) is a phospholipid residing on early endosomes, where it is proposed to be involved in endosomal fusion. We synthesized membrane-permeant derivatives of PtdIns(3)P, including a caged version that is to our knowledge the first photoactivatable phosphoinositide derivative developed so far. In living cells, photoactivation of caged PtdIns(3)P induced rapid endosomal fusion in an EEA1-dependent fashion, thus providing in vivo evidence that PtdIns(3)P is a sufficient signal for driving this process.

PMID:
20364126
DOI:
10.1038/nchembio.348
[Indexed for MEDLINE]

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