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Eukaryot Cell. 2010 May;9(5):831-3. doi: 10.1128/EC.00032-10. Epub 2010 Apr 2.

Single-step affinity purification for fungal proteomics.

Author information

1
Department of Molecular Genetics, Ohio State University, 496 W. 12th Ave., Columbus, OH 43210, USA.

Abstract

A single-step protein affinity purification protocol using Aspergillus nidulans is described. Detailed protocols for cell breakage, affinity purification, and depending on the application, methods for protein release from affinity beads are provided. Examples defining the utility of the approaches, which should be widely applicable, are included.

PMID:
20363899
PMCID:
PMC2863959
DOI:
10.1128/EC.00032-10
[Indexed for MEDLINE]
Free PMC Article

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