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Clinics (Sao Paulo). 2010 Mar;65(3):305-9. doi: 10.1590/S1807-59322010000300011.

Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma.

Author information

1
Laboratory of Molecular and Cellular Biology, Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP, Brazil. fkmiura@hotmail.com

Abstract

INTRODUCTION:

Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential.

OBJECTIVE:

To develop an experimental malignant astrocytoma model with the characteristics of the human tumor.

METHOD:

Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats.

RESULTS:

All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed.

CONCLUSION:

A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.

KEYWORDS:

Athymic Rowett rats; Brain tumor; Experimental model; U87MG cells

PMID:
20360922
PMCID:
PMC2845772
DOI:
10.1590/S1807-59322010000300011
[Indexed for MEDLINE]
Free PMC Article

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