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Mol Cell Proteomics. 2010 Aug;9(8):1634-49. doi: 10.1074/mcp.R000001-MCP201. Epub 2010 Mar 31.

Probing native protein structures by chemical cross-linking, mass spectrometry, and bioinformatics.

Author information

1
Institute of Molecular Systems Biology, Eidgenössiche Technische Hochschule (ETH) Zurich, Zurich, Switzerland.

Abstract

Chemical cross-linking of reactive groups in native proteins and protein complexes in combination with the identification of cross-linked sites by mass spectrometry has been in use for more than a decade. Recent advances in instrumentation, cross-linking protocols, and analysis software have led to a renewed interest in this technique, which promises to provide important information about native protein structure and the topology of protein complexes. In this article, we discuss the critical steps of chemical cross-linking and its implications for (structural) biology: reagent design and cross-linking protocols, separation and mass spectrometric analysis of cross-linked samples, dedicated software for data analysis, and the use of cross-linking data for computational modeling. Finally, the impact of protein cross-linking on various biological disciplines is highlighted.

PMID:
20360032
PMCID:
PMC2938055
DOI:
10.1074/mcp.R000001-MCP201
[Indexed for MEDLINE]
Free PMC Article

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