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Biotechniques. 2010 Mar;48(3):235-6. doi: 10.2144/000113368.

A lens-specific co-injection marker for medaka transgenesis.

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Department of Transcriptional Regulation, Institute of Molecular Genetics Prague, Czech Republic.


Recent advances in generating transgenic fish have improved the efficiency of germline transmission and enabled the generation of large numbers of transgenic animals. A suitable co-injection marker may help facilitate the preselection of transgenic embryos. For this purpose, a lens-specific marker appears to be a suitable candidate since the lens is a well-defined tissue that is easily accessible for examination of reporter gene expression. We constructed reporter vectors including the mouse gamma-F crystallin (mgammaF-Cry) promoter, which drives high levels of lens-specific heterologous expression of the reporter gene and thereby enables easy sorting of transgenic fish.

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