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J Assist Reprod Genet. 2010 Jun;27(6):265-76. doi: 10.1007/s10815-010-9407-6. Epub 2010 Apr 1.

Genome-wide microarray evidence that 8-cell human blastomeres over-express cell cycle drivers and under-express checkpoints.

Author information

1
Bedford Stem Cell Research Foundation, 206 Elm St, Suite 106, Somerville, MA 02144, USA. kiessling@bedfordresearch.org

Abstract

PURPOSE:

To understand cell cycle controls in the 8-Cell human blastomere.

METHODS:

Data from whole human genome (43,377 elements) microarray analyses of RNAs from normal 8-Cell human embryos were compiled with published microarrays of RNAs from human fibroblasts, before and after induced pluripotency, and embryonic stem cells. A sub database of 3,803 genes identified by high throughput RNA knock-down studies, plus genes that oscillate in human cells, was analyzed.

RESULTS:

Thirty-five genes over-detected at least 7-fold specifically on the 8-Cell arrays were enriched for cell cycle drivers and for proteins that stabilize chromosome cohesion and spindle attachment and limit DNA and centrosome replication to once per cycle.

CONCLUSIONS:

These results indicate that 8-cell human blastomere cleavage is guided by cyclic over-expression of key proteins, rather than canonical checkpoints, leading to rapidly increasing gene copy number and a susceptibility to chromosome and cytokinesis mishaps, well-noted characteristics of preimplantation human embryos.

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PMID:
20358275
PMCID:
PMC2914593
DOI:
10.1007/s10815-010-9407-6
[Indexed for MEDLINE]
Free PMC Article

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