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Lab Chip. 2010 Apr 21;10(8):1079-85. doi: 10.1039/b920883f. Epub 2010 Jan 20.

Infrared light induced patterning of proteins on ppNIPAM thermoresponsive thin films: a "protein laser printer".

Author information

1
Materials Science and Engineering, Bioengineering, Lehigh University, Bethlehem, PA 18015, USA. xuc207@lehigh.edu

Abstract

Protein micropatterns have applications in fundamental life sciences and clinical medicine. In this work, we present a new technique to create 2-D protein micropatterns by local activation of a thin film of thermoresponsive plasma-deposited poly(N-isopropylacrylamide) (ppNIPAM) using a computer-controlled infrared laser beam. While the whole substrate is exposed to the protein solution, protein deposition happens only at laser-activated locations. A few seconds of laser exposure is all that is required to form a pattern with resolution in the single micrometre range. Successful ligand binding after protein deposition indicates that protein function remains intact after laser-induced adsorption onto ppNIPAM. This rapid, simple technique advances currently available strategies for protein patterning by its potential to pattern proteins in an enclosed environment or onto a 3-D scaffold.

PMID:
20358117
DOI:
10.1039/b920883f
[Indexed for MEDLINE]

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