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Biochemistry. 2010 May 4;49(17):3511-3. doi: 10.1021/bi100430s.

Na(+):aspartate coupling stoichiometry in the glutamate transporter homologue Glt(Ph).

Author information

1
Department of Biochemistry, University of Groningen, Groningen Biomolecular Science and Biotechnology Institute, Nijenborgh 4, 9747 AG Groningen, The Netherlands.

Abstract

The Na(+) aspartate symporter Glt(Ph) from Pyrococcus horikoshii is the only member of the glutamate transporter family for which crystal structures have been determined. The cation:aspartate coupling stoichiometry is unknown, thus hampering the elucidation of the ion coupling mechanism. Here we measure transport of (22)Na(+) and [(14)C]aspartate in proteoliposomes containing purified Glt(Ph) and demonstrate that three Na(+) ions are symported with aspartate.

PMID:
20349989
DOI:
10.1021/bi100430s
[Indexed for MEDLINE]

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