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Wei Sheng Wu Xue Bao. 2010 Jan;50(1):7-14.

[16S rRNA gene in community structure analysis and the optimized amplification--a review].

[Article in Chinese]

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State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.


The purpose of this review was to offer reference for the study of community analysis in which 16S rRNA was taken as target molecule. We summarized the information of 16S rRNA including its function in cells, transcription organization, the position of variable regions and conserved regions, copies numbers of 16S rRNA gene, secondary structure, some artifacts generated during PCR amplification and the solution to avoid the artifacts.

[Indexed for MEDLINE]
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