The aim of this study was to determine the prevalence of toxoplasmosis, listeriosis and leishmaniasis in dogs in Diyarbakir region, Turkey. A total of 100 sera were collected from healthy dogs and tested for toxoplasmosis, leishmaniasis and listeriosis by the Sabin-Feldman Dye Test (SFDT), Indirekt Florescence Antikor Test (IFAT) and, Osebold Agglutination Test (OAT), respectively. Among these 100 dogs, 94 (94%) were seropositive for toxoplasmosis and and 17 (17%), for listeriosis. All of them were found to be seronegative for leishmaniasis. No statistically significant differences were observed between male and female dogs in the seroprevalence of toxoplasmosis and listeriosis. As a result, the presence of anti-Toxoplasma gondii and Listeria monocytogenesis specific antibodies in dogs in the region of Diyarbakir was determined.