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J Biomed Biotechnol. 2010;2010:101932. doi: 10.1155/2010/101932. Epub 2010 Mar 21.

SAROTUP: scanner and reporter of target-unrelated peptides.

Author information

1
Key Laboratory for Neuroinformation of Ministry of Education, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China. hj@uestc.edu.cn

Abstract

As epitope mimics, mimotopes have been widely utilized in the study of epitope prediction and the development of new diagnostics, therapeutics, and vaccines. Screening the random peptide libraries constructed with phage display or any other surface display technologies provides an efficient and convenient approach to acquire mimotopes. However, target-unrelated peptides creep into mimotopes from time to time through binding to contaminants or other components of the screening system. In this study, we present SAROTUP, a free web tool for scanning, reporting and excluding possible target-unrelated peptides from real mimotopes. Preliminary tests show that SAROTUP is efficient and capable of improving the accuracy of mimotope-based epitope mapping. It is also helpful for the development of mimotope-based diagnostics, therapeutics, and vaccines.

PMID:
20339521
PMCID:
PMC2842971
DOI:
10.1155/2010/101932
[Indexed for MEDLINE]
Free PMC Article

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