Format

Send to

Choose Destination
Chem Biol. 2010 Mar 26;17(3):236-42. doi: 10.1016/j.chembiol.2010.02.012.

Aptazyme-mediated regulation of 16S ribosomal RNA.

Author information

1
Department of Chemistry, Konstanz Research School Chemical Biology and the Zukunftskolleg, University of Konstanz, 78457 Konstanz, Germany.

Abstract

Developing artificial genetic switches in order to control gene expression via an external stimulus is an important aim in chemical and synthetic biology. Here, we expand the application range of RNA switches to the regulation of 16S rRNA function in Escherichia coli. For this purpose, we incorporated hammerhead ribozymes at several positions into orthogonalized 16S rRNA. We observed that ribosomal function is remarkably tolerant toward the incorporation of large additional RNA fragments at certain sites of the 16S rRNA. However, ribozyme-mediated cleavage results in severe reduction of 16S rRNA stability. We carried out an in vivo screen for the identification of sequences acting as ligand-responsive RNA switches, enabling thiamine-dependent switching of 16S rRNA function. In addition to expanding the regulatory toolbox, the presented artificial riboswitches should prove valuable to study aspects of rRNA folding and stability in bacteria.

PMID:
20338515
DOI:
10.1016/j.chembiol.2010.02.012
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center