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BMC Biochem. 2010 Mar 25;11:14. doi: 10.1186/1471-2091-11-14.

Interaction of connexin43 and protein kinase C-delta during FGF2 signaling.

Author information

1
Department of Orthopaedics, University of Maryland, School of Medicine, Baltimore, MD, USA.

Abstract

BACKGROUND:

We have recently demonstrated that modulation of the gap junction protein, connexin43, can affect the response of osteoblasts to fibroblast growth factor 2 in a protein kinase C-delta-dependent manner. Others have shown that the C-terminal tail of connexin43 serves as a docking platform for signaling complexes. It is unknown whether protein kinase C-delta can physically interact with connexin43.

RESULTS:

In the present study, we investigate by immunofluorescent co-detection and biochemical examination the interaction between Cx43 and protein kinase C-delta. We establish that protein kinase C-delta physically interacts with connexin43 during fibroblast growth factor 2 signaling, and that protein kinase C delta preferentially co-precipitates phosphorylated connexin43. Further, we show by pull down assay that protein kinase C-delta associates with the C-terminal tail of connexin43.

CONCLUSIONS:

Connexin43 can serve as a direct docking platform for the recruitment of protein kinase C-delta in order to affect fibroblast growth factor 2 signaling in osteoblasts. These data expand the list of signal molecules that assemble on the connexin43 C-terminal tail and provide a critical context to understand how gap junctions modify signal transduction cascades in order to impact cell function.

PMID:
20338032
PMCID:
PMC2855512
DOI:
10.1186/1471-2091-11-14
[Indexed for MEDLINE]
Free PMC Article

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