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Anal Chem. 2009 Aug 15;81(16):7087-90. doi: 10.1021/ac900968r.

Determination of bacterial antibiotic resistance based on osmotic shock response.

Author information

1
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.

Abstract

We investigate the buoyant mass of bacterial cells in real time with the suspended microchannel resonator (SMR) as the population recovers from an osmotic shock. The density of the culture medium is chosen such that the bacteria initially have a positive buoyant mass which becomes negative as they recover from the hyperosmotic stress. This behavior can be used to differentiate between an antibiotic-resistant and an antibiotic-susceptible strain of the pathogenic bacteria Citrobacter rodentium, and we propose a general approach for exploiting the high precision of the SMR for rapid detection of antibiotic resistance.

PMID:
20337387
PMCID:
PMC4031755
DOI:
10.1021/ac900968r
[Indexed for MEDLINE]
Free PMC Article

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