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Arch Virol. 2010 May;155(5):789-93. doi: 10.1007/s00705-010-0645-9. Epub 2010 Mar 24.

Protein kinase C-dependent phosphorylation of Borna disease virus P protein is required for efficient viral spread.

Author information

1
Department of Virology, Institute for Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Strasse 11, 79104, Freiburg, Germany.

Abstract

Mutational analysis of the phosphate acceptor sites of the Borna disease virus (BDV) phosphoprotein (P) has suggested a role of phosphorylation for viral spread. However, the studied mutant viruses also had two amino acid exchanges in the X protein, because the reading frames of P and X overlap. To determine the relative contribution of P and X to viral attenuation, we studied a P variant with serine-to-leucine substitutions (P(S26L,S28L)) in which the wild-type X sequence was conserved. Viral spread of rBDV-P(S26L,S28L) was impaired in human oligodendroglioma cells and in adult rats. Thus, BDV-P phosphorylation contributes to efficient viral dissemination.

PMID:
20333534
DOI:
10.1007/s00705-010-0645-9
[Indexed for MEDLINE]

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