Send to

Choose Destination
Microsurgery. 2010 May;30(4):275-81. doi: 10.1002/micr.20779.

Improvement of the skin flap survival with the bone marrow-derived mononuclear cells transplantation in a rat model.

Author information

Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.


Partial necrosis of skin flaps remains a significant problem in plastic and reconstructive surgery. In this study we attempted to evaluate the effect of bone marrow-derived mononuclear cells (BM-MNCs) transplantation on improvement of skin flap survival in a rat random pattern skin flap model. Thirty Wistar rats were divided into three groups with each consisting of 10 rats. BM-MNCs and the adipose-derived stem cells (ADSCs) were transplanted into the subcutaneous tissue in the area where the flap would be dissected. The flaps were then raised two days after cells transplantation. The animals receiving the preoperative Dulbecco's Modified Eagle Medium (DMEM) treatment were used as the controls. On the 7th postoperative day, the survival areas of flaps were measured and tissues were collected for examinations. The results showed that the mean survival areas were 46.33 +/- 13.46% in the ADSCs group and 50.06 +/- 13.82% in the BM-MNCs group as the percentages of the total skin flaps, which were significantly higher than that in the control group (26.33 +/- 7.14%) (P < 0.05). Histological analysis showed increased neovascularization in the flap treated with BM-MNCs when compared with ADSCs transplantation. Survival BM-MNCs and ADSCs were detected in the flap tissues. Higher levels of the basic fibroblast growth factor (bFGF) and vascular endothelium growth factor (VEGF) were found in the BM-MNCs transplantation group (P < 0.05). The findings from this study demonstrated that preoperative treatment with BM-MNCs transplantation could promote neovascularization and improve flap survival. These effects of BM-MNCs on flap survival were comparable with ADSCs transplantation, but without necessity of in vitro cells expansion.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center