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Appl Microbiol Biotechnol. 2010 May;86(5):1281-92. doi: 10.1007/s00253-010-2524-4. Epub 2010 Mar 23.

Single-cell analysis and isolation for microbiology and biotechnology: methods and applications.

Author information

1
Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan. anaerobe@mail.ecc.u-tokyo.ac.jp

Abstract

Various single-cell isolation techniques, including dilution, micromanipulation, flow cytometry, microfluidics, and compartmentalization, have been developed. These techniques can be used to cultivate previously uncultured microbes, to assess and monitor cell physiology and function, and to screen for novel microbiological products. Various other techniques, such as viable staining, in situ hybridization, and those using autofluorescence proteins, are frequently combined with these single-cell isolation techniques depending on the purpose of the study. In this review article, we summarize currently available single-cell isolation techniques and their applications, when used in combination with other techniques, in microbiological and biotechnological studies.

PMID:
20309540
DOI:
10.1007/s00253-010-2524-4
[Indexed for MEDLINE]

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