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Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6526-31. doi: 10.1073/pnas.0913154107. Epub 2010 Mar 22.

Imaging extrasynaptic glutamate dynamics in the brain.

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1
Departments of Pharmacology and Neurobiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.

Abstract

Glutamate is the major neurotransmitter in the brain, mediating point-to-point transmission across the synaptic cleft in excitatory synapses. Using a glutamate imaging method with fluorescent indicators, we show that synaptic activity generates extrasynaptic glutamate dynamics in the vicinity of active synapses. These glutamate dynamics had magnitudes and durations sufficient to activate extrasynaptic glutamate receptors in brain slices. We also observed crosstalk between synapses--i.e., summation of glutamate released from neighboring synapses. Furthermore, we successfully observed that sensory input from the extremities induced extrasynaptic glutamate dynamics within the appropriate sensory area of the cerebral cortex in vivo. Thus, the present study clarifies the spatiotemporal features of extrasynaptic glutamate dynamics, and opens up an avenue to directly visualizing synaptic activity in live animals.

PMID:
20308566
PMCID:
PMC2851965
DOI:
10.1073/pnas.0913154107
[Indexed for MEDLINE]
Free PMC Article
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