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Nucleic Acids Res. 1977 Dec;4(12):4165-74.

End labeling of enzymatically decapped mRNA.

Abstract

A method is presented for rapid and efficient 5' end labeling with 32P of capped mRNAs, by a series of three enzymatic reactions: the blocking nucleotide of the cap structure is removed by tobacco acid pyrophosphatase, and after dephosphorylation with alkaline phosphatase the 5' end is labeled with gamma-32-P-ATP and T4 polynucleotide kinase.

PMID:
202926
PMCID:
PMC343232
DOI:
10.1093/nar/4.12.4165
[Indexed for MEDLINE]
Free PMC Article

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