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Methods Mol Biol. 2010;638:189-200. doi: 10.1007/978-1-60761-611-5_14.

Biochemical methods used to study the gene expression and protein complexes in the filamentous fungus Neurospora crassa.

Author information

1
Department of Physiology, The University of Texas Southwestern Medical Center, Dallas, TX, USA.

Abstract

Biochemical approaches are powerful tools for investigating mechanisms of biological processes. Here, we describe several biochemical approaches that have been successfully in our laboratory to study the filamentous fungus Neurospora crassa. These approaches include protein extraction and western blot analysis, protein purification using epitope-tagged fusion protein, protein immunoprecipitation (IP) and Chromatin Immunoprecipitation (ChIP) assays. These methods can also be modified for use in other filamentous fungi.

PMID:
20238270
DOI:
10.1007/978-1-60761-611-5_14
[Indexed for MEDLINE]

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