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Methods Mol Biol. 2010;638:3-19. doi: 10.1007/978-1-60761-611-5_1.

Protoplast transformation of filamentous fungi.

Author information

1
Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, NY, USA.

Abstract

The protoplast method for the transformation of filamentous fungi is described in detail, as is the Restriction Enzyme-Mediated Integration (REMI) procedure for introducing tagged mutations into the fungal genome. A split marker method for generating PCR fragments for targeted integration and deletion of genes of interest is also detailed.

PMID:
20238257
DOI:
10.1007/978-1-60761-611-5_1
[Indexed for MEDLINE]

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