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BMC Microbiol. 2010 Mar 17;10:83. doi: 10.1186/1471-2180-10-83.

Invasion of Ureaplasma diversum in Hep-2 cells.

Author information

1
Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo. Av. Professor Lineu Prestes, 1374. 05508-900, São Paulo, SP, Brazil.

Abstract

BACKGROUND:

Understanding mollicutes is challenging due to their variety and relationship with host cells. Invasion has explained issues related to their opportunistic role. Few studies have been done on the Ureaplasma diversum mollicute, which is detected in healthy or diseased bovine. The invasion in Hep-2 cells of four clinical isolates and two reference strains of their ureaplasma was studied by Confocal Laser Scanning Microscopy and gentamicin invasion assay.

RESULTS:

The isolates and strains used were detected inside the cells after infection of one minute without difference in the arrangement for adhesion and invasion. The adhesion was scattered throughout the cells, and after three hours, the invasion of the ureaplasmas surrounded the nuclear region but were not observed inside the nuclei. The gentamicin invasion assay detected that 1% of the ATCC strains were inside the infected Hep-2 cells in contrast to 10% to the clinical isolates. A high level of phospholipase C activity was also detected in all studied ureaplasma.

CONCLUSIONS:

The results presented herein will help better understand U. diversum infections, aswell as cellular attachment and virulence.

PMID:
20236540
PMCID:
PMC2907839
DOI:
10.1186/1471-2180-10-83
[Indexed for MEDLINE]
Free PMC Article

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