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J Am Chem Soc. 2010 Aug 11;132(31):10628-9. doi: 10.1021/ja101387b.

Rapid visualization and large-scale profiling of bacterial lipoproteins with chemical reporters.

Author information

1
Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, New York 10065, USA.

Abstract

Lipoproteins are a largely uncharacterized class of proteins in bacteria. In this study, metabolic labeling of bacteria with fatty acid chemical reporters allowed rapid profiling of lipid-modified proteins. We identified many candidate lipoproteins in Escherichia coli and detected a novel modification on YjgF. This chemical approach should facilitate future characterization of lipoproteins.

PMID:
20230003
PMCID:
PMC3031127
DOI:
10.1021/ja101387b
[Indexed for MEDLINE]
Free PMC Article
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