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Cell Biol Toxicol. 2010 Oct;26(5):457-67. doi: 10.1007/s10565-010-9156-4. Epub 2010 Mar 14.

Astaxanthin prevents in vitro auto-oxidative injury in human lymphocytes.

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1
Cellular Physiology Laboratory, Postgraduate Program-Health Science, CBS, Cruzeiro do Sul University, Tatuapé, São Paulo, Brazil.

Abstract

Upon mitogen sensitization, lymphocytes undergo proliferation by oxyradical-based mechanisms. Through continuous resting-restimulation cycles, lymphocytes accumulate auto-induced oxidative lesions which lead to cell dysfunction and limit their viability. Astaxanthin (ASTA) is a nutritional carotenoid that shows notable antioxidant properties. This study aims to evaluate whether the in vitro ASTA treatment can limit oxyradical production and auto-oxidative injury in human lymphocytes. Activated lymphocytes treated with 5 microM ASTA showed immediate lower rates of O(2)(*-) /H(2)O(2) production whilst NO* and intracellular Ca(2+) levels were concomitantly enhanced (<or=4 h). In long-term treatments (>24 h), the cytotoxicity test for ASTA showed a sigmoidal dose-response curve (LC50 = 11.67 +/- 0.42 microM), whereas higher activities of superoxide dismutase and catalase in 5 microM ASTA-treated lymphocytes were associated to significant lower indexes of oxidative injury. On the other hand, lower proliferative scores of ASTA lymphocytes might be a result of diminished intracellular levels of pivotal redox signaling molecules, such as H(2)O(2). Further studies are necessary to establish the ASTA-dose compensation point between minimizing oxidative damages and allowing efficient redox-mediated immune functions, such as proliferation, adhesion, and oxidative burst.

PMID:
20229275
DOI:
10.1007/s10565-010-9156-4
[Indexed for MEDLINE]
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