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Biotechnol Lett. 2010 Jul;32(7):957-61. doi: 10.1007/s10529-010-0239-3. Epub 2010 Mar 13.

Retinal production from beta-carotene by beta-carotene 15,15'-dioxygenase from an unculturable marine bacterium.

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1
Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul, 143-701, Republic of Korea.

Abstract

The conversion of beta-carotene to retinal by a recombinant beta-carotene 15,15'-dioxygenase (Blh protein) from an unculturable marine bacterium was optimized in aqueous solution. Toluene was optimal solvent for the dissolution of beta-carotene and the optimal solution for the conversion reaction contained 2.4% (w/v) Tween 20, 0.15 U enzyme/ml, and 350 mg beta-carotene/l. Under these conditions, the enzyme produced 181 mg retinal/l after 20 h. This is the highest reported value for the retinal concentration from beta-carotene.

PMID:
20229064
DOI:
10.1007/s10529-010-0239-3
[Indexed for MEDLINE]
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