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Am J Surg. 2010 Mar;199(3):377-80; discussion 380-1. doi: 10.1016/j.amjsurg.2009.09.012.

Protective effect of methylprednisolone on warm ischemia-reperfusion injury in a cholestatic rat liver.

Author information

1
Department of Surgery, Providence Hospital and Medical Centers, Southfield, MI, USA.

Abstract

BACKGROUND:

Cholestasis has been identified as a risk factor for oxidative stress, and it potentially enhances after ischemic-reperfusion injury. The aim of this study was to evaluate the role of methylprednisolone on warm ischemia-reperfusion injury in the presence of cholestasis.

METHODS:

A reversible cholestatic rat model was created. After 7 days, rats received 30 mg/kg of intravenous methylprednisolone 2 hours before ischemia, followed by 30 minutes of ischemia. Rats were euthanized 24 hours after ischemia. Serum aspartate aminotransferase and interleukin-6 were measured, and the liver was harvested for histology and myeloperoxidase estimation.

RESULTS:

Methylprednisolone had a protective effect, with a statistically significant decrease in aspartate aminotransferase (P=.01) and a trend toward decreased levels of interleukin-6 (P=.07). Histology showed a significant difference in architectural distortion (P=.01), cytoplasmic vacuolation (P=.01), and nodular hepatocellular necrosis (P=.04).

CONCLUSIONS:

Methylprednisolone attenuated the ischemic-reperfusion injury in the presence of cholestasis and can be considered for clinical use in the presence of cholestasis.

PMID:
20226914
DOI:
10.1016/j.amjsurg.2009.09.012
[Indexed for MEDLINE]

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