Abstract
Escherichia coli delta cya-283 is a 75-bp in-frame deletion overlapping the 5' end of delta cya-854; delta cya-201 is a 41-bp frameshift deletion overlapping the 3' end of delta cya-854. Sequence repeats were found at the boundaries of delta cya-283 and delta cya-201, suggesting a mechanism for deletion formation. Recombinant DNA procedures were used to construct a strain in which the total cya structural gene in the chromosome was replaced by the kanamycin resistance gene.
MeSH terms
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Adenylyl Cyclases / genetics*
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Base Sequence
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Chromosome Deletion
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DNA Mutational Analysis
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DNA, Bacterial / genetics
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Genes, Bacterial
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Genetic Vectors
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Molecular Sequence Data
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Polymerase Chain Reaction
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Restriction Mapping
Substances
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DNA, Bacterial
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Adenylyl Cyclases
Associated data
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GENBANK/M55488
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GENBANK/M55489
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GENBANK/M55490
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GENBANK/M55491
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GENBANK/M55492
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GENBANK/M55493
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GENBANK/M55494
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GENBANK/M81169
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GENBANK/X01653
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GENBANK/X52418