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Methods Mol Biol. 2010;587:45-56. doi: 10.1007/978-1-60327-355-8_4.

Kinetics of motor protein translocation on single-stranded DNA.

Author information

1
Department of Physics and Astronomy, University of Kansas, Lawrence, KS, USA.

Abstract

The translocation of nucleic acid motor proteins along DNA or RNA can be studied in ensemble experiments by monitoring either the kinetics of the arrival of the protein at a specific site on the nucleic acid filament (generally one end of the filament) or the kinetics of ATP hydrolysis by the motor protein during translocation. The pre-steady state kinetic data collected in ensemble experiments can be analyzed by simultaneous global non-linear least squares (NLLS) analysis using a simple sequential "n-step" mechanism to obtain estimates of the rate-limiting step(s) in the translocation cycle, the average "kinetic step-size," and the efficiency of coupling ATP binding and hydrolysis to translocation.

PMID:
20225141
PMCID:
PMC3775657
DOI:
10.1007/978-1-60327-355-8_4
[Indexed for MEDLINE]
Free PMC Article

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