Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR

J Clin Microbiol. 2010 May;48(5):1884-7. doi: 10.1128/JCM.02540-09. Epub 2010 Mar 10.

Abstract

The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

MeSH terms

  • Amino Acid Substitution / genetics
  • Antiviral Agents / pharmacology
  • Drug Resistance, Viral*
  • Humans
  • Influenza A Virus, H1N1 Subtype / drug effects*
  • Influenza A Virus, H1N1 Subtype / genetics
  • Influenza A Virus, H1N1 Subtype / isolation & purification
  • Influenza, Human / virology*
  • Molecular Sequence Data
  • Mutation, Missense*
  • Neuraminidase / genetics*
  • Oseltamivir / pharmacology
  • RNA, Viral / genetics
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sequence Analysis, DNA
  • Time Factors
  • Viral Proteins / genetics*
  • Virology / methods*

Substances

  • Antiviral Agents
  • RNA, Viral
  • Viral Proteins
  • Oseltamivir
  • NA protein, influenza A virus
  • Neuraminidase

Associated data

  • GENBANK/GU371269