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J Proteomics. 2010 May 7;73(7):1321-33. doi: 10.1016/j.jprot.2010.02.011. Epub 2010 Feb 26.

Characterization of potential allergens in fenugreek (Trigonella foenum-graecum) using patient sera and MS-based proteomic analysis.

Author information

1
National Veterinary Institute, Oslo, Norway. christiane.faste@vetinst.no

Abstract

BACKGROUND:

Fenugreek is a legume plant used as an ingredient of curry spice. Incidents of IgE-mediated food allergy to fenugreek have been reported. Coincidence with allergy to peanut, a major food allergen, seems to be common suggesting a rather high rate of cross-reactivity.

OBJECTIVE:

Characterization of fenugreek allergens using patient sera and mass spectrometry-based proteomic analysis.

METHODS:

Allergenic fenugreek proteins were detected by immunoblotting, using sera from 13 patients with specific IgE to peanut and fenugreek. IgE-binding proteins were analyzed by peptide mass fingerprinting and peptide sequencing.

RESULTS:

A fenugreek protein quintet in the range from 50 kDa to 66 kDa showed high IgE-affinity, the protein at 50 kDa reaching the strongest signals in all patients. Proteomic analyses allowed the classification of several fenugreek proteins to a number of allergen families. Fenugreek 7S-vicilin and 11S-legumin were partly sequenced and revealed considerable homologies to peanut Ara h 1 and Ara h 3, respectively. The presence of a fenugreek 2S albumin and pathogenesis-related (PR-10) plant pollen protein was assumed by database searching results.

CONCLUSION:

In this study, individual fenugreek proteins were characterised for the first time. Observed homologies to major peanut allergens provide a molecular explanation for clinical cross-reactivity.

PMID:
20219717
PMCID:
PMC2894366
DOI:
10.1016/j.jprot.2010.02.011
[Indexed for MEDLINE]
Free PMC Article

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