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Technol Cancer Res Treat. 2010 Apr;9(2):139-47.

Profiling DNA methylomes from microarray to genome-scale sequencing.

Author information

1
The Ohio State University Comprehensive Cancer Center, Columbus, OH 43210, USA. Yi-wen.huang@osumc.edu

Abstract

DNA cytosine methylation is a central epigenetic modification which plays critical roles in cellular processes including genome regulation, development and disease. Here, we review current and emerging microarray and next-generation sequencing based technologies that enhance our knowledge of DNA methylation profiling. Each methodology has limitations and their unique applications, and combinations of several modalities may help build the entire methylome. With advances on next-generation sequencing technologies, it is now possible to globally map the DNA cytosine methylation at single-base resolution, providing new insights into the regulation and dynamics of DNA methylation in genomes.

PMID:
20218736
PMCID:
PMC3011833
DOI:
10.1177/153303461000900203
[Indexed for MEDLINE]
Free PMC Article

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