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Curr Pharm Biotechnol. 2010 Apr;11(3):267-71.

A highly controllable reconstituted cell-free system--a breakthrough in protein synthesis research.

Author information

1
The Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, FSB-401, 5-1-5 Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

Abstract

The PURE system is a highly controllable cell-free protein synthesis system composed of individually prepared components that are required for protein synthesis in Escherichia coli. The PURE system contains neither nucleases nor proteases, both of which degrade DNA or mRNA templates and proteins. The protein products are easily purified using affinity chromatography to remove the tagged protein factors. The PURE system should help to create new fields in protein research.

PMID:
20210745
DOI:
10.2174/138920110791111889
[Indexed for MEDLINE]

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