[Study of methylation of promoter of EYA1 gene in microtia]

Zhonghua Zheng Xing Wai Ke Za Zhi. 2009 Nov;25(6):436-9.
[Article in Chinese]

Abstract

Objective: To explore the methylation of CpG islands in promoter of eye absent gene 1 (EYA1) in microtia.

Methods: The methylation of CpG islands in EYA1 gene in 64 microtias and 36 healthy controls were measured using the technique of matrix-assisted laser desorption/ionization-time of flight.

Results: The methylation of CpG_Unit3 and CpG_Unit5 of EYA1 gene in microtia were 0.09258 +/- 0.033846 and 0.0922 +/- 0.02379, respectively, which were significantly lower than those in control.

Conclusions: Hypomethylation in microtia may be related to the pathogenesis of the disease.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Child
  • Child, Preschool
  • CpG Islands*
  • DNA Methylation*
  • Ear / abnormalities*
  • Female
  • Humans
  • Intracellular Signaling Peptides and Proteins / genetics*
  • Male
  • Middle Aged
  • Nuclear Proteins / genetics*
  • Promoter Regions, Genetic
  • Protein Tyrosine Phosphatases / genetics*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Young Adult

Substances

  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • EYA1 protein, human
  • Protein Tyrosine Phosphatases