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Nat Methods. 2010 Apr;7(4):307-9. doi: 10.1038/nmeth.1438. Epub 2010 Mar 7.

Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions.

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1
Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.

Abstract

We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.

PMID:
20208532
PMCID:
PMC2850821
DOI:
10.1038/nmeth.1438
[Indexed for MEDLINE]
Free PMC Article
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