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Biochem Biophys Res Commun. 2010 Apr 2;394(2):348-53. doi: 10.1016/j.bbrc.2010.03.009. Epub 2010 Mar 4.

Quantification of cellular uptake and in vivo tracking of transduction using real-time monitoring.

Author information

1
Division of Bio-Imaging, Chuncheon Center, Korea Basic Science Institute, Chuncheon, Kangwon-Do 200-701, Republic of Korea.

Abstract

Protein transduction domains (PTDs) are short amino acid sequences that promote their own translocation across the cell plasma membrane and have been studied for possible use in drug delivery and gene therapy. However, no direct method to quantify transduction is available. Here, using a new luciferase-tagged human PTD, we show that cellular uptake levels can be determined in a reliable manner. Furthermore, we show that enhanced in vivo tracking by human PTD can be quantified in a mouse model. This is the first report on the direct quantification of PTD transduction in vitro and in vivo, which will be necessary for studying its possible therapeutic application in drug delivery and gene therapy.

PMID:
20206600
DOI:
10.1016/j.bbrc.2010.03.009
[Indexed for MEDLINE]

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